安利佳

姓名:安利佳 电子邮箱地址:bioeng@dlut.edu.cn 主要学历及工作经历: 1982年2月于辽宁师范学院获理学学士学位 1984年10月于东北师范大学遗传学专业获理学硕士学位 1989年6月于东北师范大学植物学专业获理学博士学位。 1984年10月至1998年3月在辽宁师范大学任教，1996年晋升教授。 1998年3月，调入大连理工大学任教至今，曾任生物工程系主任，研究所所长。 1995年获辽宁省优秀青年科技工作者称号；1996年获辽宁省首届青年科研人才基金；1999年获大连市优秀人才奖；2001年获大连市优秀专家称号。2001年被评为辽宁省第二批百千万人才工程百人层次人选。2004年担任辽宁省生物技术协会专家委员会专家 主要学术及社会兼职: 中国遗传学会理事 教育部科委生命科学学部委员 辽宁省生物技术协会专家委员会专家。 研究领域（研究课题）: 1. 植物转基因工程与育种技术平台研究。编号：JY03-B-18，国家科技部植物转基因重大专项，100万元，2003-2004 2. 基因工程关键技术。项目编号：2001101001，辽宁省科技厅，210万元，2001-2004 3. 无载体、无标记基因植物转基因技术。项目编号：2003B4NS015，大连市科技局，100万元，2003-2004 4. 蛇岛蝮蛇类凝血酶基因的分离和表达。辽宁省科技攻关，10万元，1999.01-2001.12 5. 蛇岛蝮蛇类凝血酶基因的分离和表达。大连市科委，15万元，1998-2001 6. 胸腺素α1基因工程药物开发。通化金马制药股份公司，390万元，1998.01~2001.12 7. 杨树基因工程育种。鞍山生生高新植物技术应用股份有限公司，25万元，2001.9-2003.9 8. 长春花碱单体的分离研究。大连理工大学领先集团有限公司，20万元，2002.7-2003.7 9. 真福康保健功效的验证与鉴定。大连理工大学领先集团有限公司，10万元，2002.7-2003.7 指导硕、博士生研究方向: 植物基因工程 生物制药 出版著作和论文: 五年来共发表文章120余篇，SCI收录42篇，主要文章包括： 1. Properties of A. ficuum AS3.324 phytase expressed in tobacco. Process Biochemistry, 2005,40:213–216 SCI 2. Purification, characterization and gene cloning of a novel phospholipase A2 from the venom of Agkistrodon blomhoffii ussurensis. The International Journal of Biochemical & Cell Biology. 2005,37:558-565 SCI 3. Neuroprotective properties of catalpol in transient global cerebral ischemia in gerbils: dose-response, therapeutic time-window and long-term efficacy. Brain Research, 2004,1029:179-185 SCI 4. Plasmodium falciparum histone acetyltransferase, a yeast GCN5 homologue involved in chromatin remodeling. Eukaryotic Cell, 2004,3(2):264-276 5. Catalpol inhibits apoptosis in hydrogen peroxide-induced PC12 cells by preventing cytochrome c release and inactivating of caspase casecade. Toxicon, 2004,43:53-59 6. PfADA2, a Plasmodium falciparum homologue of the transcriptional coactivator ADA2 and its in vivo association with the histone acetyltransferase PfGCN5. Gene, 2004,336:251-261 7. Construction of Targeting Vector and Expression of Green Fluorescent Protein in the Silkworm, Antheraea pernyi. DNA and Cell Biology. 2003,22:441-446 8. The Expression of GFP Under the Control of Fibroin Promotor in Primary ovarian cells of Antheraea pernyi. Journal of Biosciences, 2003,28(6):691-695 9. 菟丝子提取物在PC12细胞株中的神经营养因子样活性。生物化学与生物物理进展。2003,30(2):226-230 10. Codonopsis pilosula (Franch) Nannf total alkaloids potentiate neurite outgrowth induced by nerve growth factor in PC12 cells. Acta Pharmacologica Sinica, 2003,24(9):913-917 11. Effect of Cuscuta chinensis glycoside on the neuronal differentiation of rat pheochromocytoma PC12 cells. International Journal of Developmental Neuroscience, 2003,21:277-281 12. Hydrogen peroxide-induced apoptosis in PC12 cells and the protective effect of puerarin. Cell Biology International, 2003,27:1025-1031 13. Neuroprotective effect of Alpinia oxyphylla Miq. fruits against glutamate-induced apoptosis in cortical neurons. Toxicology Letters, 2003,144(2):205–212 14. Neuroprotective effect of Alpinia oxyphylla extract against glutamate excitotoxicity in cultured mouse cortical neurons. Neurosci Res Commun, 2003,33(2):105–113 15. Investigation of the neuronal death mode induced by glutamate treatment in serum-, antioxidant-free primary cultured cortical neurons. Developmental Brain Research, 2003,145(2):263-268 16. A new way for chemical degradation of plastic by natural volatile constituents of Ledum palustre. Chinese Science Bulletin, 2003,48(16):1718-1721 17. Enhancing ethanol tolerance of a self-flocculating fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae by Mg2+ via reduction in plasma membrane permeability. Biotechnology Letters, 2003,25:1191-1194 18. Dextromethorphan protects dopaminergic neurons against inflammation-mediated degeneration through inhibition of microglial activation. The Journal Of Pharmacology and Experimental Therapeutics, 2003,305(1):212-218 19. 酸性水溶液中硫氰酸钼(V)配合物的研究。光谱学与光谱分析，2003,23(2)337-339 20. Purification and characterization of ulva pertusa kjellm alkaline phosphatase. Preparative Biochemistry & Biotechnology, 2003,33(2):113-123 21. Cloning of cDNA Encoding Choline Monooxygenase from Suaeda liaotungensis and Salt Tolerance of Transgenic Tobacco. Acta Botanica Sinica, 2003,45(2):242-247 22. Molecular cloning and characterization of betaine aldehyde dehydrogenase gene from Suaeda liaotungensis and its use in improved tolerance to salinity in transgenic tobacco. Biotechnol Lett. 2003,25(17):1431-1436 23. Characterization of Sporothric schenckii by random amplication of polymorphic DNA assay. Chinese Medicine Journal, 2003,116(2):239-242 24. Expression of gloshedobin, a thrombin-like enzyme from the venom of Gloydius shedaoensis, in Escherichia coli. Biotechnology Letters, 2003,25(2):101–104 25. High-level expression of a soluble snake venom enzyme, gloshedobin, in E. coli in the presence of metal ions. Biotechnology Letters, 2003,25(8):607–610 26. Purification and characterization of ginsenoside--arabinofuranase hydrolyzing ginsenoside Rc into Rd from the fresh root of Panax ginseng. Process Biochemistry, 2002,37:793-798 27. Cloning and Expression of defibrase cDNA from the venom of Gloydius shedaoensis. Biotechnology Letters, 2002,24:135-138 28. Cloning Expression and Purification of Gussrobin A Thrombin-like Enzyme from the Snake Venom of Gloydius ussuriensis. Prog Biochem Biophys. 2002,34(1):6-10 29. Expression, Purification and Partial Characterization of Recombinant Defibrase, a Thrombin-Like Enzyme from the Venom of Glyodius shedaoensis. Prog Biochem Biophys. 2002,29(3)390-393 30. Production of a Functional Single-chain Fv Protein in Transgenic Tobacco Root Exudates. Biotechnology letters. 2002,24(18):1531-1534 31. An expeditious method for constructing T-vectors using Eam 1105 Ⅰ cassettes. Plant Molecular Biology Reporter, 2002,20:189a-189e 32. Application of DNA sequencing in detection of telomerase activity. Biochemical Engineering Journal. 2002,11(2-3):79-85 33. Production of high affinity human single-chain antibody against preS1 of Hepatitis B Virus: comparison of large naïve and in vitro immune phage displayed antibody library. Progressing in Biochemistry and Biophysics. 2002,29(4):572-575 34. Inhibition by naloxone stereoisomers of beta-amyloid peptide (1-42)-induced superoxide production in microglia and degeneration of cortical and mesencephalic neurons. Journal of Pharmacology and Experimental Therapeutics, 2002,302(3):1212-1219 35. A Serum- and Antioxidant-free Primary Culture Model of Mouse Cortical Neurons for Pharmacological Screen and Studies of Neurotrophic and Neuroprotective Agents. Cellular and Molecular Neurobiology. 2002,22(2):197-206 36. Expression of endoplasmic reticulum molecular chaperon GRP94 in human lung cancer tissues and its clinical significance. Chinese Medical Journal, 2002,115(11):1615-1619 37. Embryonic stem cell as nuclear donor could promote in vitro development of the heterogeneous reconstructed embryo. Chinese Science Bulletin, 2002,47(21):1811-1815 38. 铜锌超氧化物歧化酶与色氨酸钴(Ⅱ)的相互作用。光谱学与光谱分析，2000,3:305-307 39. 紫外-可见吸收光谱法测定配合显色反应的焓变。光谱学与光谱分析，2001,21(4):527-528 40. Kinetics of Inactivation of Ulva pertusa Kjellm Alkaline Phosphatase by Ethylenediaminetetraacetic Acid Disodium. Journal of Enzyme Inhibition, 2001,16:313-319 41. Purification and Characterization of ginsenoside--Glucosidase from Ginseng. Chem. Pharm. Bull, 2001,49(7):795-798 42. 分光光度法研究EDTA对小牛肠碱性磷酸酶的不可逆抑制动力。光谱学与光谱分析，2001,21(5):701-703 43. 碱性磷酸梅与Cu (II) 相互作用的谱学研究。光谱学与光谱分析，2001,21(4):432-434 44. 氨基酰化酶与Cu(Ⅱ)相互作用的谱学研究。光谱学与光谱分析，2001,21(6):804-806 45. 可见光谱学研究核酸酶P1与氯化铜(Ⅱ)的相互作用。光谱学与光谱分析，2000,20(3):308-310 46. 大连蛇岛蝮蛇类凝血酶基因克隆与表达研究。大连理工大学学报，2003,43(2):156-159 47. 新的重组蛋白表达系统——植物根分泌和叶分泌。生物化学与生物物理进展，2003,30(1):128 48. 抗乙肝病毒表面抗原PreS1(20-47)的单链抗体在转基因烟草根分泌物中的初步表达。高技术通讯，2003,13(9):35-38 49. Variation and characterization analysis of partial fragment of fibroin gene from silkworm, Antheraea pernyi. High Technology Letters, 2003,9(3):29-32 50. 喜树碱生物合成途径及其代谢调控的研究进展。高技术通讯，2004,14(5):106-110 51. 菟丝子提取物对PC12细胞中GAP-43表达影响。大连理工大学学报，2004,44(3):378-382 52. Rapid Amplification of 5′cDNA End of Suaeda liaotungensis Choline Monooxygenase Using Inverse PCR RACE. High Technol Lett 2002,8(1):5-7 53. Separation and purification of betaine aldehyde dehydrogenase from wild Suaeda liaotungensis. High Technology Letters, 2002,8(2):5-17 54. Cloning a Full-length cDNA Encoding UDP-glucose Pyrophosphorylase from Amorpha Fruticosa by PCR-Based Methods. High Technology Letters, 2002,8(3):5-10 55. An Lijia. A co-expression system based on phage and phagemid to select cognate antibody-antigen pairs in vivo. High Technology Letters, 2002,8(2):5-10 56. Construction of Large Human Single-chain Antibody Phage Display Library. High Technology Letters, 2002,8(3):1-4 57. 精子干细胞转染法制备转基因兔的研究。高技术通讯，2001,11(130):17-21 58. 反向嵌套PCR高效扩增辽宁碱蓬甜菜硷醛脱氢酶cDNA 5’末端序列。高技术通讯，2001,1(11):17-19 59. Research on telomerase detection of activity by combining DNA sequence analysis with TRAP. High Technology Letters, 2001,7(3):8-10 60. 新型染料配基对碱性磷酸酶的亲合纯化。高校化学工程学报，2001,5(6):563-567 61. Separation and Purification of Thrombin-like Enzyme by Affinity Adsorbents. High Technology Letters，2001,7(4):13-15 62. 卵黄免疫球蛋白配基亲和纯化碱性磷酸酶。中国农业科学，2004,37(6):908-911 科研成果及所受奖励: 共承担国家、省、市课题10项，项目经费总计900万元； 申报国内外专利13项，其中国际专利1项； 由安利佳教授为负责人完成的“一种植物分子育种新技术的建立与应用”项目鉴定会，于2004年12月19日通过了辽宁省科技厅科技成果鉴定，达到了国际领先水平。 所主持的辽宁省科技厅项目“基因工程关键技术”和大连市科技局项目“无载体、无标记基因植物转基因技术”于2004年4月6日通过了辽宁省科技厅科技成果验收和大连市科技局科技成果鉴定，达到了国际先进水平。 2004年安利佳教授率领的课题组被学校评为大连理工大学科研创新团队。 在读硕士、博士人数: 在读博士30人，硕士18人 已毕业硕士博士人数: 已指导毕业博士19人，硕士16人 以上资料最后修改时间: 2005-4-3